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Next Generation Sequencing

The Center for Integrated Biosytems offers next generation sequencing services on the Illumina NextSeq, and Illumina MiSeq. Applications include whole genome sequencing, RNA-Seq, methylation analysis, amplicon sequencing, genotyping by sequencing (GBS), microRNA sequencing, 16S ribosomal sequencing, ChIP-Seq, targeted sequencing, and more. We also offer size selection on the Blue Pippin automated size selection platform.

NextSeq

Services


Blue Pippin size selection

The Blue Pippin is capable of selecting 100-50,000 bp fragments. However, we do not routinely have the supplies for size selection above 600 bp. Please contact us if you need selection of fragments above 600 bp. Samples are $15 each. Subsequent analysis on the TapeStation is an additional $5 per sample.

 

Library Preparation

We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.

DNA library (e.g. whole genome)              $90

RNA-Seq                                                  $110

microRNA                                                 $130

 

Sequencing

While we strive to achieve the best results possible, the listed number of reads is the specification published by Illumina and individual runs may vary from these numbers.

The listed read length can be changed if necessary. Please contact us if you need something different.

 

Illumina MiSeq

 

Internal USU Price

External Price

Expected read number

Typical read length

MiSeq Reagent Kit v3 (150 cycle)

$1,225

$1,720

25M

2X75

MiSeq Reagent Kit v3 (600 cycle)

$1,940

$2,715

25M

2X300

MiSeq Reagent Kit v2 (50 cycle)

$1,125

$1,575

15M

1x50

MiSeq Reagent Kit v2 (300 cycle)

$1,385

$1,945

15M

2X150

MiSeq Reagent Kit v2 (500 cycle)

$1,530

$2,145

15M

2X250

MiSeq Reagent Micro Kit v2 (300 cycle)

$635

$970

4M

2X150

MiSeq Reagent Nano Kit v2 (300 cycle)

$500

$740

1M

2X150

MiSeq Reagent Nano Kit v2 (500 cycle)

$560

$830

1M

2X250

 

 

Illumina NextSeq

 

Internal USU Price

External Price

Expected read number

Typical read length

NextSeq Mid Output Kit v2 (150 cycles)

$1,425

$1,995

130M

2X75

NextSeq Mid Output Kit v2 (300 cycles)

$2,170

$3,040

130M

2X150

NextSeq High Output Kit v2 (75 cycles)

$1,880

$2,630

400M

1X75

NextSeq High Output Kit v2 (150 cycles)

$3,420

$4,790

400M

2X75

NextSeq High Output Kit v2 (300 cycles)

$5,360

$7,500

400M

2X150