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Next Generation Sequencing

The Center for Integrated Biosytems offers next generation sequencing services on the Illumina NextSeq, Illumina MiSeq, and Ion Torrent PGM. Applications include whole genome sequencing, RNA-Seq, methylation analysis, amplicon sequencing, genotyping by sequencing (GBS), microRNA sequencing, 16S ribosomal sequencing, ChIP-Seq, targeted sequencing, and more.


Library Preparation:

We offer standard library preparation for RNA-Seq, microRNA, and whole genome sequencing samples. For other sequencing types, we can assist you with designing your protocol and can work with you as needed to sequence your samples.

DNA library (e.g. whole genome)                 $90

RNA-Seq                                                      $110

microRNA                                                     $130

Sequencing:

While we strive to achieve the best results possible, the listed number of reads is the specification published by Illumina and individual runs may vary from these numbers.

The listed read length can be changed if necessary. Please contact us if you need something different.

Illumina MiSeq

 

Internal USU Price

External Price

Expected Read Number

Typical Read Length

 MiSeq Reagent Kit v3 (150 cycle)

$1,030

$1,440

25M

2X75

 MiSeq Reagent Kit v3 (600 cycle)

$1,660

$2,300

25M

2X300

 MiSeq Reagent Kit v2 (50 cycle)

$950

$1,320

15M

1x50

 MiSeq Reagent Kit v2 (300 cycle)

$1,175

$1,640

15M

2X150

 MiSeq Reagent Kit v2 (500 cycle)

$1,300

$1,820

15M

2X250

 MiSeq Reagent Micro Kit v2 (300 cycle)

$570

$795

4M

2X150

 MiSeq Reagent Nano Kit v2 (300 cycle)

$430

$600

1M

2X150

 MiSeq Reagent Nano Kit v2 (500 cycle)

$480

$670

1M

2X250

 

Illumina NextSeq

 

Internal USU Price

External Price

Expected read number

Typical read length

 NextSeq Mid Output Kit v2 (150 cycles)

$1,200

$1,620

130M

2X75

 NextSeq Mid Output Kit v2 (300 cycles)

$1,860

$2,510

130M

2X150

 NextSeq High Output Kit v2 (75 cycles)

$1,600

$2,160

400M

1X75

 NextSeq High Output Kit v2 (150 cycles)

$2,990

$4,000

400M

2X75

 NextSeq High Output Kit v2 (300 cycles)

$4,730

$6,300

400M

2X150

 

Ion Torrent PGM

 

Internal USU Price

External Price

Expected Read Number

Maximum Read Length

 PGM 314

$630

$850

600 K

400

 PGM 316

$830

$1120

3 M

400

 PGM 318

$1030

$1390

5.5 M

400

 

Data Analysis:

Data analysis is available through the CIB Bioinformatics Core Facility.